β-galactosidase mRNA (unmodified)
β-Galactosidase is a glycoside hydrolase enzyme that catalyzes the hydrolysis of terminal, non-reducing β-D-galactose residues in β-D-galactosides. The β-galactosidase assay is widely used in genetics, molecular biology, and other life science fields. The activity of β-galactosidase can be detected using X-gal, a synthetic substrate that produces a characteristic blue dye upon cleavage by the enzyme.
Croyez’s β-Galactosidase mRNA was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.
Croyez’s β-Galactosidase mRNA was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.
mRNA Length:
3443 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
3443 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
Figure 1. β-galactosidase mRNA activity assay
Figure 2. Capillary Electrophoresis
β-galactosidase mRNA was analyzed by capillary electrophoresis
Figure 3. β-galactosidase mRNA was analyzed on a 1% TAE agarose gel at 100 V for 30 minutes
